Quantification of choline uptake with [11C]choline PET

[Methyl-11C]Choline ([11C]choline) is a marker for cellular proliferation, because choline is a precursor for the biosynthesis of phospholipids which are the essential components of all cell membranes (Krohn, 2001). Choline is transported into the cell, phosphorylated, and converted to phosphatidylcholine.

Input function

Intact tracer represents 62% ± 19% of the total radioactivity in arterial plasma at 5 min after injection and 27% ± 12% at 15 min; the major metabolite in plasma is [11C]betaine (Roivainen et al., 2000).

Tumours

Usefulness of [11C]choline in the tumour diagnosis has been demonstrated (Hara 2002), and quantification by using Patlak plot (MTGA for irreversibly binding tracers), and SUV in clinical setting have been validated (Utriainen et al., 2003; Sutinen et al., 2004). However, cell proliferation may not explain the uptake of [11C]choline in certain cancers (Breeuwsma et al., 2005). 11C- and 18F-labelled choline are well-suited to imaging tumours of bladder and at the pelvic region, because the radioactivity in urine is minimal.

Atherosclerosis

11C- and 18F-labelled choline uptake is increased in activated macrophages. Animal models have shown potential usefulness in imaging atherosclerosis (Matter et al., 2006; Laitinen et al., 2010; Hellberg et al., 2016).

Rheumatoid arthritis

[11C]Choline has been shown to accumulate in clinically active synovitis in . The uptake (measured as SUV and net influx rate, Ki) correlated with the volume of synovium (Roivainen et al., 2003). Initial uptake of [11C]choline is very fast, and the concentration of C-11 label reaches a plateau 10 min after bolus injection (Roivainen et al., 2003; Roivainen & Yli-Kerttula, 2006), suggesting irreversible kinetics during the PET study. Patlak plot was linear (Roivainen et al., 2003), confirming the irreversible uptake. Furthermore, Patlak plot intercept with y axis was close to zero (Roivainen et al., 2003), suggesting that calculation of Patlak plot (requiring dynamic PET scanning) could quantitatively be replaced by FUR, which only requires one late PET scan and enables whole-body imaging.


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References:

Breeuwsma AJ, Pruim J, Jongen MM, Suurmeijer AJ, Vaalburg W, Nijman RJ, de Jong IJ. In vivo uptake of [11C]choline does not correlate with cell proliferation in human prostate cancer. Eur J Nucl Med Mol Imaging 2005; 32(6):668-673.

Hara T. 11C-Choline and 2-deoxy-2-[18F]fluoro-D-glucose in tumor imaging with positron emission tomography. Mol Imaging Biol. 2002; 4(4): 267-273.

Krohn KA. Evaluation of alternative approaches for imaging cellular growth. Q J Nucl Med. 2001; 45: 174-178.

Roivainen A, Forsback S, Grönroos T, Lehikoinen P, Kähkönen M, Sutinen E, Minn H. Blood metabolism of [methyl-11C]choline; implications for in vivo imaging with positron emission tomography. Eur J Nucl Med. 2000; 27(1): 25-32.

Roivainen A, Parkkola R, Yli-Kerttula T, Lehikoinen P, Viljanen T, Möttönen T, Nuutila P, Minn H. Use of positron emission tomography with methyl-11C-choline and 2-18F-2-deoxy-D-glucose in comparison with magnetic resonance imaging for the assessment of inflammatory proliferation of synovium. Arthr Rheum. 2003; 48(11): 3077-3084.

Roivainen A, Yli-Kerttula T. Whole-body distribution of 11C-choline and uptake in knee synovitis. Eur J Nucl Med Mol Imaging 2006; 33(11): 1372-1373.

Sutinen E, Nurmi M, Roivainen A, Varpula M, Tolvanen T, Lehikoinen P, Minn H. Kinetics of [11C]choline uptake in prostate cancer: a PET study. Eur J Nucl Med Mol Imaging 2004; 31(3): 317-324.

Utriainen M, Komu M, Vuorinen V, Lehikoinen P, Sonninen P, Kurki T, Utriainen T, Roivainen A, Kalimo H, Minn H. Evaluation of brain tumor metabolism with [11C]choline PET and 1H-MRS. J Neurooncol. 2003; 62(3): 329-338.



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Created at: 2007-12-03
Updated at: 2018-08-11
Written by: Vesa Oikonen