Perfusion (blood flow)

Perfusion is the volume of blood flowing through certain mass (or volume) of tissue per unit time. Blood flow is usually given in units mL/(100 g * min) or mL/(mL * min).

Tissue perfusion can be measured noninvasively with positron emission tomography using the following general techniques:

Tracers that remain in vascular space cannot be used to measure blood flow (Lassen, 1984). At its best, such tracer can provide an estimate of plasma or blood volume in the tissue, which may vary in the same direction as the plasma flow, and thus appear to correlate with blood flow.

The methods to measure perfusion with diffusible and inert tracers are based on the principle of exchange of inert gas between blood and tissues (Kety and Schmidt, 1945), and on the Fick’s principle. 133Xe clearance method has been widely used, but it will only give an estimate of the total blood flow (both nutritive and non-nutritive) from all the tissues that is drained by the vein, and the tissue-blood -partition coefficient must be known (but is highly variable).

Nutritive vs nonnutritive perfusion

When perfusion is measured using diffusible PET tracers, such as [15O]H2O, the nonnutritive (noneffective) fraction of blood flow (blood flowing through shunts or other tissues, like skin) is not included in the perfusion estimate. By definition, in shunts arterial and venous blood concentrations are equal, Ca - Cv = 0, and thus it has no effect on the concentration in tissue, based on the Fick’s principle.

However, nonnutritive blood flow will increase the estimate of arterial blood volume, because both the arterial and venous fraction of the shunt volume will have the same kinetics.

Microspheres of different diameters can be used in animal studies to measure nutritive and nonnutritive blood flow.

See also:


Huang S-C, Carson RE, Phelps ME. Measurement of local blood flow and distribution volume with short-lived isotopes: a general input technique. J Cereb Blood Flow Metab. 1982; 2: 99-108.

Jones SC, Greenberg JH, Dann R, Robinson GD Jr, Kushner M, Alavi A, Reivich M. Cerebral blood flow with the continuous infusion of oxygen-15-labeled water. J Cereb Blood Flow Metab. 1985; 5: 566-575.

Kety SS, Schmidt CF. The determination of cerebral blood flow in man by the use of nitrous oxide in low concentrations. Am J Physiol. 1945; 143: 53-66.

Lassen NA. Cerebral transit of an intravascular tracer may allow measurement of regional blood volume but not regional blood flow. J Cereb Blood Flow Metab. 1984; 4: 633-634.

LeBlanc AD, Riley RC, Robinson RG. Simultaneous measurement of total and nutritional coronary blood flow in dogs. Circulation 1974; 49(2): 338-347.

Sharp PF. The measurement of blood flow in humans using radioactive tracers. Physiol Meas. 1994; 15: 339-379.

Traystman RJ. The paper that completely altered our thinking about cerebral blood flow measurement. J Appl Physiol. 2004; 97: 1601-1602.

Zierler KL. Equations for measuring blood flow by external monitoring of radioisotopes. Circ Res. 1965; 16: 309-321.

Tags: ,

Created at: 2014-04-07
Updated at: 2017-03-14
Written by: Vesa Oikonen