Radioactive metabolites of PET tracers
PET scanner can measure the total amount of radioactivity in tissue, but it does not provide information on the chemical form of molecule(s) in which the radioactive label was attached at the time of the PET scan. PET tracers are metabolized in the body, and some of the products will carry the radioactive label. Some of these reactions are planned, for example the phosphorylation and subsequent trapping of [18F]FDG, but usually the PET tracers are metabolized into undesirable radioactive compounds that will confound the quantification. Radioactive metabolites in the plasma samples can be corrected, but the metabolites in the tissue must be accounted for in the analysis model. Tracers for brain studies are by design molecules which do not metabolize into radioactive products that could cross the blood-brain barrier (Ma et al., 2010), although there are important exceptions to this rule, such as [18F]FDOPA.
Identification of metabolites
Radioactive metabolites must be identified for two purposes: First, for the determination of the fractions of parent tracer in plasma, and secondly, for ruling out or appropriately accounting for the tissue uptake of the radioactive metabolites.
Circulating metabolites of PET radioligands are mainly produced in the liver. Hepatocytes and hepatic microsome preparations are therefore used to study the metabolism in vitro (Giron et al., 2008; Amini et al., 2013), already during the tracer development phase. Metabolic profiles are species dependent.
- PET data
- Correction of plasma TAC for metabolites
- Processing input data
- Fractions of authentic tracer in plasma
- Chromatographic methods for measurement of metabolite fractions in plasma
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Created at: 2015-12-14
Updated at: 2015-12-15
Written by: Vesa Oikonen