PET imaging of serotonin system
Serotonin (5-hydroxytryptamine, 5-HT) is a monoamine neurotransmitter. In gastrointestinal tract 5-HT regulates smooth muscle tone; enterochromaffin (EC) cells contain >90% of the total serotonin in the human body (Beattie and Smith, 2008). Tryptophan hydroxylase catalyses the first and rate-limiting step of synthesis of serotonin, converting L-tryptophan, one of the essential amino acids in humans, into 5-hydroxy-L-tryptophan (5-HTP). There are two isoforms of tryptophan hydroxylase, THP1 in the peripheral tissues including pineal gland, and THP2 expressed in the brain and enteric nervous system. 5-HTP is then converted to serotonin by aromatic L-amino acid decarboxylase (AADC, AAAD, 5-hydroxytryptophan decarboxylase). Serotonin synthesis rate has been studied using [11C]AMT and [11C]5-HTP. Blood platelets (thrombocytes) contain serotonin transporter and are able to store high concentrations of serotonin from the gastrointestinal tract, releasing it as vasoconstrictor from dense storage granules. Bone contains 5-HT receptors, and serotonin levels affect the bone mass.
Serotonergic neurons in the central nervous system (CNS) are also able to synthesize 5-HT. 5-HT cannot cross the BBB. Serotonergic neurons are mainly located in the dorsal raphe nucleus, from where the axons extend to other parts of the CNS, including the cerebellum and spinal cord. In the CNS serotonin regulates the mood, perception, reward, aggression, appetite, attention, etc, and is therefore involved amongst other things in anxiety and panic disorders, depression and appetite. There seems to be a link between sex hormone levels and serotonin signalling (Perfalk et al., 2017). Lysergic acid diethylamide (LSD) had a central role in discovering the serotonergic system and its involvement in the CNS disorders (L´Estrade et al., 2018). 5-HT is cleared from the synaptic cleft mainly by serotonin transporter (SERT). Serotonin reuptake inhibitors (SSRIs) are used as antidepressants. Inside the cell, 5-HT is further transported into synaptic vesicles by monoamine transporter 2 (VMAT2). 5-HT can also be degraded by MAO-A, located at mitochondrial membranes, to 5-hydroxyindoleatic acid (5-HIAA) by glial cells. 5-HIAA passes to the extracellular space and is then actively transported away from the CNS.
Serotonin receptors are categorized into seven families, 5-HT1 - 5-HT7 comprising at least 16 distinct mammalian subtypes. 5-HT3 class is ionotropic (ligand-gated cation channel), other serotonin receptors are G-protein coupled receptors.
5-HT1A receptor density is high in limbic brain regions, such as hippocampus, lateral septum, cortical areas, and raphe nuclei, but very low in the basal ganglia and cerebellum (Barnes and Sharp, 1999). 5-HT1A receptors have been successfully studied using [11C]WAY-100635, [18F]MPPF, [18F]FCWAY, and [11C]CUMI-101 (Paterson et al, 2013). [18F]MPPF has also shown promise in quantification of the endogenous serotonin concentration. Agonist tracer [18F]F13640 may provide information on the high affinity state 5-HT1ARs (Vidal et al., 2018).
For 5-HT1B [11C]AZ10419369 and [11C]P943 have been used. 5-HT1BR is found especially in the basal ganglia.
5-HT2A receptors can be studied using [18F]altanserin, [18F]deuteroaltanserin, and [11C]MDL100907. [11C]Cimbi-36 may overestimate the density of 5-HT2ARs in regions with high 5-HT2CR density (Ettrup et al., 2016). 5-HT2AR density is high in cortical areas, caudate nucleus, nucleus accumbens, and hippocampus. 5-HT2AR is also found in peripheral neurons and inflammatory cells.
5-HT6 imaging has been conducted using [11C]GSK215083. Highest 5-HT6R concentrations in the CNS are found in the striatum and nucleus accumbens, and lesser concentrations in amygdala, hypothalamus, thalamus, hippocampus, and cerebral cortex.
Serotonin transporter (SERT, 5-HTT) belongs to a family of neurotransmitter symporters. SERT and NET can take up extracellular dopamine, too, especially in the Parkinsonian striatum when dopamine transporters (DATs) are reduced. Several polymorphisms of the SERT are associated with interindividual differences in serotonergic system and predisposition to depression, anxiety disorders, and alcohol dependence.
β-[123I]CIT has similar affinity for SET and DAT, and it has been used to study SERT in the midbrain where SERT is abundant as compared to DAT. [11C]McN 5652 had too slow binding kinetics in the midbrain and high nonspecific binding.
5-HT synthesis and release
5-HT synthesis rate can be quantified using [11C]AMT and [11C]HTP (Paterson et al, 2013).
Quantification of serotonin release in the brain has been difficult (Paterson et al., 2013; Tyacke and Nutt, 2015), probably because of very active autoregulation, although some success has been reported using for example [18F]altanserin.
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Created at: 2016-08-23
Updated at: 2018-05-21
Written by: Vesa Oikonen