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A brief comparison of PET analysis methods

Single-bolus studies

Table 1. Comparison of analysis methods for single-bolus PET studies
Method Outcome Requirements for Applicable to parametric imaging Vulnerabilities
PET scan Input
Fit to compartment model
(gold standard)
or
reference region model
Transport and binding/metabolism rate constants, vascular volume, VT, Ki Dynamic study arterial plasma no, except K1, Ki, or VT images None, if comprehensive model. All plasma input methods are vulnerable to systematic errors in plasma metabolite analysis. Possibly non-specific binding in plasma (fP)
BPND, R1 Dynamic study reference tissue yes/no (depending on model) non-specific binding in tissue (free fraction fND)
Transport and binding/metabolism rate constants, vascular volume, volumes of distribution (VT), BPF, BPP, BPND, R1 Dynamic study arterial plasma and reference tissue yes/no (depending on model) possibly non-specific binding in plasma and/or tissue (fP and fND)
Spectral analysis Ki or VT, number of identifiable compartments Dynamic study plasma yes non-specific binding in plasma and/or tissue (fP and fND)
Ratio BPND Dynamic study reference yes non-specific binding in tissue (fND), vascular volume, bias dependent on BP
Ratio, approaches BPND Single scan reference yes time from injection, non-specific binding in tissue (fND), vascular volume
Multiple-time graphical analysis (MTGA): Gjedde-Patlak and Logan plots Ki Dynamic study plasma yes non-specific binding to plasma proteins (fP)
Ki Dynamic study reference yes non-specific binding in tissue (fND)
VT Dynamic study plasma yes non-specific binding in plasma and tissue (fP and fND)
DVR (VT/VND) Dynamic study reference yes non-specific binding in tissue (fND), reference k2
Fractional uptake rate, sometimes referred to as Retention index FUR, approaches Ki Single scan plasma yes Distribution volumes of free and non-specifically bound tracer, vascular volume
Standardized uptake value SUV Single scan i.d. yes Perfusion, peripheral clearance, non-specific binding in plasma and tissue (fP and fND), vascular volume

The outcome of many of the methods is the (equilibrium) volume of distribution VT (or DV). If valid reference region exists, the regional distribution volume ratio can be calculated as DVR = DVROI/DVREFERENCE. This, in turn, relates to the binding potential BPND: BPND = DVR - 1. However, this measure is vulnerable to change of non-specific binding in tissue.

Bolus + infusion studies

Table 2. Comparison of analysis methods for bolus/infusion PET studies
Method Outcome Requirements for Applicable to parametric imaging Vulnerabilities
PET scan Input
Ratio BPND Single scan reference yes non-specific binding in tissue (fND), vascular volume
VT Single scan venous plasma yes non-specific binding in tissue (fND), vascular volume


References:

Innis RB, Cunningham VJ, Delforge J, Fujita M, Gjedde A, Gunn RN, Holden J, Houle S, Huang SC, Ichise M, Iida H, Ito H, Kimura Y, Koeppe RA, Knudsen GM, Knuuti J, Lammertsma AA, Laruelle M, Logan J, Maguire RP, Mintun MA, Morris ED, Parsey R, Price JC, Slifstein M, Sossi V, Suhara T, Votaw JR, Wong DF, Carson RE. Consensus nomenclature for in vivo imaging of reversibly binding radioligands. J Cereb Blood Flow Metab. 2007; 27(9): 1533-1539.

Logan J, Alexoff D, Kriplani A. Simplifications in analyzing positron emission tomography data: effects on outcome measures. Nucl Med Biol. 2007; 34: 743-756.

Slifstein M, Laruelle M. Models and methods for derivation of in vivo neuroreceptor parameters with PET and SPECT reversible radiotracers. Nucl. Med. Biol. 2001; 28: 595-608.



Last updated: 2008-11-28

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