fit_mo2 3.0.0 © 1998-2008 by Turku PET Centre

Fits the following compartmental model dedicated for skeletal muscle (1, 2, 3) to regional PET [O-15]O2 study data. The model parameters are perfusion (Flow, K1), oxygen extraction ratio (OER = K1/(k2+k3)), partition coefficients of oxygen and water (K1/k2 and pH2O), blood volume fraction (Vb) and time delay (delayT). Blood Muscle _______________________________________ | K1 | | | O ----------> O + O -Mb | | 2 <---------- 2 2 | | k2 | | | | k3 | | | V | | K1 | | | H O ----------> H O | | 2 <---------- 2 | | | K1/pH2O | |______________|________________________| Model definitions: K1=Flow (mL/min/dL), OER=k3/(k2+k3), Ki=Flow*OER (mL/min/dL), Metabolic rate of oxygen MRO2=Ki*[O2]a (mmol/min/dL) Command line arguments: 1) Blood O2 TAC file 2) Blood H2O TAC file 3) Muscle TAC file (*.dft) 4) Fit duration (sec) 5) Result file General options: -SD[=<Y|n>] Standard deviations are calculated and saved in results (y), or not calculated (n). -CL[=<Y|n>] 95% Confidence limits are calculated and saved in results (y), or not calculated (n). -svg=<Filename> Plots of original and fitted TACs are written in specified file in Scalable Vector Graphics (SVG) 1.1 format; specification in -fit=<Filename> Fitted regional TACs are written in DFT format. -h or --help Print this message and exit --version or --build Print software build information and exit --silent Program works silently, printing only error and warning messages --verbose Program prints lots of information about what it is doing Options to set model parameters: -cO2a=<Arterial O2 concentration> Set the O2 content (mL O2/100 mL blood) of arterial blood to calculate rMRO2, for example -cO2a=19.8; if not specified, program can calculate only Ki for [O-15]O2 -Af=<Arterial proportion (%)> Set the simulated arterial proportion of total blood volume; default is 30% Options for constraining model parameters: -i=<filename> Specify the constraints for model parameters; This file with default values can be created by giving this option as the only command-line argument to this program. Parameter can be fixed to a certain value by setting its lower and upper limit to that value. -Vb=<Blood volume (%)> Set the regional blood volume; default is 3.5% -pH2O=<value> Set the partition coefficient of water; default is 0.99 -Flow=<value> Set the regional perfusion (blood flow) value, e.g. based on [O-15]H2O study, in units mL/100mL/min -fK1k2 K1/k2 for oxygen is not estimated as a free parameter, but it is calculated from OER and saturation curves for hemoglobin and myoglobin -K1k2=<value> Specify the K1/k2 for oxygen. -Sao2=<value> Saturation of arterial blood hemoglobin; default is 0.97 -p50Hb=<value> Half-saturation pressure for hemoglobin; default is 3.6 kPa -p50Mb=<value> Half-saturation pressure for myoglobin; default is 0.319 kPa -nHb=<value> Hill coefficient for hemoglobin; default is 2.7 -Mb=<value> Concentration of myoglobin in muscle; default is 4.7 mg/g -Hb=<value> Concentration of hemoglobin in blood; default is 150 mg/g Example: fit_mo2 uo1235_o.kbq uo1235_w.kbq uo1235.dft 999 uo1235mo2.res Times must be in seconds in all data files. File format is specified in References: 1. Nuutila P, Peltoniemi P, Oikonen V, Larmola K, Kemppainen J, Takala T, Sipila H, Oksanen A, Ruotsalainen U, Bolli GB, Yki-Jarvinen H. Enhanced stimulation of glucose uptake by insulin increases exercise-stimulated glucose uptake in skeletal muscle in humans: studies using [15O]O2, [15O]H2O, [18F]fluoro-deoxy-glucose, and positron emission tomography. Diabetes 2000; 49:1084-1091. 2. Oikonen V, Nuutila P, Sipilä H, Tolvanen T, Peltoniemi P, Ruotsalainen U. Quantification of oxygen consumption in skeletal muscle with PET and oxygen-15 bolus. Eur J Nucl Med. 1998; 25: 1151. 3. Oikonen V. Modelling of low oxygen consumption. In: J. Knuuti, J. Rinne, P.Tenhonen (ed.), Medical Applications of Cyclotrons VIII. Abstracts of the VIII Symposium on the Medical Applications of Cyclotrons. Annales Universitatis Turkuensis D346:16, 1999. See also: o2metab, o2_p2w, fit_o2bl, b2t_mo2, rescoll Keywords: DFT, modelling, oxygen, skeletal muscle This program comes with ABSOLUTELY NO WARRANTY. This is free software, and you are welcome to redistribute it under GNU General Public License.

CC-GNU GPL Valid XHTML 1.0! Valid CSS!

Last updated 2009-06-18 12:38:23